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Click-Chemistry-Based Free Azide versus Azido Sugar Detection Enables Rapid In Vivo Screening of Glycosynthase Activity

https://doi.org/10.1021/acschembio.1c00585

Agrawal, Ayushi; Bandi, Chandra Kanth; Burgin, Tucker; Woo, Youngwoo; Mayes, Heather B.; Chundawat, Shishir P. (November 2021, ACS Chemical Biology)

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Carbohydrate-Active enZyme (CAZyme) enabled glycoengineering for a sweeter future

https://doi.org/10.1016/j.copbio.2020.09.006

Bandi, Chandra Kanth; Agrawal, Ayushi; Chundawat, Shishir PS (December 2020, Current Opinion in Biotechnology)
null (Ed.)
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Engineered Regulon to Enable Autonomous Azide Ion Biosensing, Recombinant Protein Production, and in Vivo Glycoengineering

https://doi.org/10.1021/acssynbio.0c00449

Bandi, Chandra Kanth; Skalenko, Kyle S.; Agrawal, Ayushi; Sivaneri, Neelan; Thiry, Margaux; Chundawat, Shishir P. (April 2021, ACS Synthetic Biology)
null (Ed.)
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Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica

https://doi.org/10.1186/s12934-019-1259-x

Ganesan, Vijaydev; Spagnuolo, Michael; Agrawal, Ayushi; Smith, Spencer; Gao, Difeng; Blenner, Mark (December 2019, Microbial Cell Factories)
null (Ed.)
Abstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica . In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica , discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica .
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